Journal: Cell

Article Title: Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

doi: 10.1016/j.cell.2019.09.022

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Gp210 (1:50 dilution) , Developmental Studies Hybridoma Bank , DSHB Cat#agp26.10; RRID: AB_528270.

Techniques: Recombinant, Software, Imaging

Journal: Human Cell

Article Title: Astragaloside IV alleviates senescence of vascular smooth muscle cells through activating Parkin-mediated mitophagy

doi: 10.1007/s13577-022-00758-6

Figure Lengend Snippet: AS-IV improved mitophagy and attenuated mitochondrial membrane potential loss in BLM-induced VSMCs. A After administration of AS-IV, MMP was identified by TMRM staining in BLM-induced VSMCs. Magnification, 200 × , scale bar = 50 μm. B TEM was used to observe the influence of AS-IV on the mitochondrial structure of BLM-induced VSMCs. C Flow Cytometer was used to assess the impact of AS-IV on the TMRM + fluorescent intensity in BLM-stimulated VSMCs. D P62 expression was examined by Western blot in BLM-induced VSMCs, which were given AS-IV, and the P62/TOMM20 value was determined. E expression of Parkin was measured by Western blot in BLM-induced VSMCs. LD low dose, MD medium dose, HD high dose. * P < 0.05, ** P < 0.05, *** P < 0.001

Article Snippet: The primary antibodies included p16 (Beyotime, AF1069, 1:1000), p21 (ProteinTech, 10,355–1-AP, 1:3000), DcR2 (Boster, A05136, 1:1500), P62 (ProteinTech, 18,420–1-AP, 1:3000), TOMM20 (Boster, BM4366, 1:2000), Drp1 (Abcam, ab184247, 1:1000), and Parkin (Boster, PB9307, 1:1500).

Techniques: Membrane, Staining, Flow Cytometry, Expressing, Western Blot

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Variation of initial glucose concentration in batch cultivations. a Oxygen transfer rate (OTR) and cumulative oxygen transfer (COT). The dashed horizontal lines show calculated OTR max levels of 62.3 mmol/L/h for 20 g/L initial glucose concentration and 60.3 mmol/L/h for 30 g/L initial glucose concentration in the medium; b glucose concentration and acetate concentration; c pH and osmolality (Osm); d cell dry weight (CDW) and EcFbFP fluorescence; Cultivation conditions: Wilms-MOPS-mineral medium (initial glucose in culture medium: 10/15/20/30 g/L, 200 mM MOPS), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Concentration Assay, Fluorescence

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Characterization of abrupt OTR increases in the fed-batch phase at varied initial reservoir glucose concentrations with the membrane-based fed-batch shake flask. Depiction of a OTR; b glucose concentration in the culture medium; c ammonium concentration in the culture medium; d ammonium concentration in the reservoir; e pH in the culture medium; f cell dry weight; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, blue dextran concentration: 1 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected; The arrows point to the occurrence of abrupt OTR increases

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Membrane, Concentration Assay

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Oxygen transfer rate of cultivations with increased ammonium sulfate concentrations in the culture medium in cultivations with the membrane-based fed-batch shake flask. Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with varied initial ammonium sulfate, initial reservoir glucose concentrations: 500 g/L, blue dextran concentration: 2 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; The arrows point to the occurrence of abrupt OTR increases

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Membrane, Concentration Assay

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Screening for adequate ammonium carbonate concentrations in the reservoir for the multi-component release with the membrane-based fed-batch shake flask. Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 500 g/L with varied initial ammonium carbonate in the reservoir along with 17.14 g/L K 2 HPO 4 , blue dextran concentration: 2 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; The arrows point to the occurrence of abrupt OTR increases

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Membrane, Concentration Assay

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Characterization of the multi-component fed-batch cultivations with varied initial glucose concentrations and 40 g/L ammonium carbonate in the reservoir with the membrane-based fed-batch shake flask. Depiction of a oxygen transfer rate; b ammonium concentration in the culture medium; c pH in the culture medium; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, 40 g/L ammonium carbonate, blue dextran concentration: 1 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected; The arrow points to the occurrence of an abrupt OTR increase

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Membrane, Concentration Assay

Journal: Microbial Cell Factories

Article Title: Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

doi: 10.1186/s12934-018-0917-8

Figure Lengend Snippet: Cell dry weight results for fed-batch cultivations with varied reservoir compositions. a Biomass concentrations with glucose fed-batch cultivations; b biomass concentrations with glucose fed-batch cultivations along with 40 g/L ammonium carbonate; all measured CDW values have been volume-corrected; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, blue dextran concentration: 1 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected

Article Snippet: Depiction of (a ) Eight replicates within one experiment; (b) Five replicates from independent experiments; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250 g/L, blue dextran concentration: 4 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD 600 : 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm 2 ; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.

Techniques: Concentration Assay, Membrane